1DOY

1H AND 15N SEQUENTIAL ASSIGNMENT, SECONDARY STRUCTURE AND TERTIARY FOLD OF [2FE-2S] FERREDOXIN FROM SYNECHOCYSTIS SP. PCC 6803

Summary for 1DOY

• Entry DOI: 10.2210/pdb1doy/pdb
• Descriptor: FERREDOXIN [2FE-2S], FE2/S2 (INORGANIC) CLUSTER (2 entities in total)
• Functional Keywords: iron-sulfur protein, electron transport
• Biological source: Synechocystis sp.
• Total number of polymer chains: 1
• Total formula weight: 10412.86

Authors

Lelong, C.,Setif, P.,Bottin, H.,Andre, F.,Neumann, J.M. (deposition date: 1995-09-14, release date: 1996-03-08, Last modification date: 2024-06-05)

Primary citation

Lelong, C.,Setif, P.,Bottin, H.,Andre, F.,Neumann, J.M.
1H and 15N NMR sequential assignment, secondary structure, and tertiary fold of [2Fe-2S] ferredoxin from Synechocystis sp. PCC 6803.
Biochemistry, 34:14462-14473, 1995

PubMed Abstract: The [2Fe-2S] ferredoxin extracted from Synechocystis sp. PCC 6803 was studied by 1H and 15N nuclear magnetic resonance. Sequence-specific 1H and 15N assignment of amino acid residues far from the paramagnetic cluster (distance higher than 8 A) was performed. Interresidue NOE constraints have allowed the identification of several secondary structure elements: one beta sheet composed of four beta strands, one alpha helix, and two alpha helix turns. The analysis of interresidue NOEs suggests the existence of a disulfide bridge between the cysteine residues 18 and 85. Such a disulfide bridge has never been observed in plant-type ferredoxins. Structure modeling using the X-PLOR program was performed with or without assuming the existence of a disulfide bridge. As a result, two structure families were obtained with rms deviations of 2.2 A. Due to the lack of NOE connectivities resulting from the paramagnetic effect from the [2Fe-2S] cluster, the structures were not well resolved in the region surrounding the [2Fe-2S] cluster, at both extremities of the alpha helix and the C and N terminus segments. In contrast, when taken separately, the beta sheet and the alpha helix were well defined. This work is the first report of a structure model of a plant-type [2Fe-2S] Fd in solution.

PubMed: 7578051
DOI: 10.1021/bi00044a024

Experimental method

SOLUTION NMR