1DOC

THE MOBIL FLAVIN OF 4-OH BENZOATE HYDROXYLASE: MOTION OF A PROSTHETIC GROUP REGULATES CATALYSIS

1DOC の概要

• エントリーDOI: 10.2210/pdb1doc/pdb
• 分子名称: P-HYDROXYBENZOATE HYDROXYLASE, BROMIDE ION, FLAVIN-ADENINE DINUCLEOTIDE, ... (5 entities in total)
• 機能のキーワード: oxidoreductase
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45545.93

構造登録者

Gatti, D.L.,Palfey, B.A.,Lah, M.S.,Entsch, B.,Massey, V.,Ballou, D.P.,Ludwig, M.L. (登録日: 1994-09-06, 公開日: 1994-11-30, 最終更新日: 2024-02-07)

主引用文献

Gatti, D.L.,Palfey, B.A.,Lah, M.S.,Entsch, B.,Massey, V.,Ballou, D.P.,Ludwig, M.L.
The mobile flavin of 4-OH benzoate hydroxylase.
Science, 266:110-114, 1994

PubMed Abstract: Para-hydroxybenzoate hydroxylase inserts oxygen into substrates by means of the labile intermediate, flavin C(4a)-hydroperoxide. This reaction requires transient isolation of the flavin and substrate from the bulk solvent. Previous crystal structures have revealed the position of the substrate para-hydroxybenzoate during oxygenation but not how it enters the active site. In this study, enzyme structures with the flavin ring displaced relative to the protein were determined, and it was established that these or similar flavin conformations also occur in solution. Movement of the flavin appears to be essential for the translocation of substrates and products into the solvent-shielded active site during catalysis.

PubMed: 7939628

実験手法

X-RAY DIFFRACTION (2 Å)