1DN0

STRUCTURE OF THE FAB FRAGMENT FROM A HUMAN IGM COLD AGGLUTININ

1DN0 の概要

• エントリーDOI: 10.2210/pdb1dn0/pdb
• 分子名称: IGM-KAPPA COLD AGGLUTININ (LIGHT CHAIN), IGM-KAPPA COLD AGGLUTININ (HEAVY CHAIN) (3 entities in total)
• 機能のキーワード: fab, igm, antibody, cold agglutinin, human, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 96871.63

構造登録者

Cauerhff, A.,Braden, B.,Carvalho, J.G.,Leoni, J.,Polikarpov, I.,Goldbaum, F. (登録日: 1999-12-15, 公開日: 2001-01-24, 最終更新日: 2024-11-06)

主引用文献

Cauerhff, A.,Braden, B.C.,Carvalho, J.G.,Aparicio, R.,Polikarpov, I.,Leoni, J.,Goldbaum, F.A.
Three-dimensional structure of the Fab from a human IgM cold agglutinin.
J.Immunol., 165:6422-6428, 2000

PubMed Abstract: Cold agglutinins (CAs) are IgM autoantibodies characterized by their ability to agglutinate in vitro RBC at low temperatures. These autoantibodies cause hemolytic anemia in patients with CA disease. Many diverse Ags are recognized by CAs, most frequently those belonging to the I/i system. These are oligosaccharides composed of repeated units of N:-acetyllactosamine, expressed on RBC. The three-dimensional structure of the Fab of KAU, a human monoclonal IgM CA with anti-I activity, was determined. The KAU combining site shows an extended cavity and a neighboring pocket. Residues from the hypervariable loops V(H)CDR3, V(L)CDR1, and V(L)CDR3 form the cavity, whereas the small pocket is defined essentially by residues from the hypervariable loops V(H)CDR1 and V(H)CDR2. This fact could explain the V(H)4-34 germline gene restriction among CA. The KAU combining site topography is consistent with one that binds a polysaccharide. The combining site overall dimensions are 15 A wide and 24 A long. Conservation of key binding site residues among anti-I/i CAs indicates that this is a common feature of this family of autoantibodies. We also describe the first high resolution structure of the human IgM C(H)1:C(L) domain. The structural analysis shows that the C(H)1-C(L) interface is mainly conserved during the isotype switch process from IgM to IgG1.

PubMed: 11086081

実験手法

X-RAY DIFFRACTION (2.28 Å)