1DGU

HOMOLOGY-BASED MODEL OF CALCIUM-SATURATED CIB (CALCIUM-AND INTEGRIN-BINDING PROTEIN)

1DGU の概要

• エントリーDOI: 10.2210/pdb1dgu/pdb
• 関連するPDBエントリー: 1DGV
• 分子名称: CALCIUM-SATURATED CIB (1 entity in total)
• 機能のキーワード: helical, ef-hands, blood clotting
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Lipid-anchor: Q99828
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20980.49

構造登録者

Hwang, P.M.,Vogel, H.J. (登録日: 1999-11-25, 公開日: 1999-12-08, 最終更新日: 2024-05-22)

主引用文献

Hwang, P.M.,Vogel, H.J.
Structures of the platelet calcium- and integrin-binding protein and the alphaIIb-integrin cytoplasmic domain suggest a mechanism for calcium-regulated recognition; homology modelling and NMR studies.
J.Mol.Recog., 13:83-92, 2000

PubMed Abstract: Calcium- and integrin-binding protein (CIB) binds to the 20-residue alphaIIb cytoplasmic domain of platelet alphaIIbbeta3 integrin. Amino acid sequence similarities with calmodulin (CaM) and calcineurin B (CnB) allowed the construction of homology-based models of calcium-saturated CIB as well as apo-CIB. In addition, the solution structure of the alphaIIb cytoplasmic domain in 45% aqueous trifluoroethanol was solved by conventional two-dimensional NMR methods. The models indicate that the N-terminal domain of CIB possesses a number of positively charged residues in its binding site that could interact with the acidic carboxy-terminal LEEDDEEGE sequence of alphaIIb. The C-terminal domain of CIB seems well-suited to bind the sequence WKVGFFKR, which forms a well-structured alpha helix; this is analogous to calmodulin and calcineurin B, which also bind alpha helices. Similarities between the C-terminal domains of CIB and calmodulin suggest that binding of CIB to the cytoplasmic domain of alphaIIb may be affected by fluctuations in the intracellular calcium concentration.

PubMed: 10822252
DOI: 10.1002/(SICI)1099-1352(200003/04)13:2<83::AID-JMR491>3.3.CO;2-1

実験手法

SOLUTION NMR