1DGP

ARISTOLOCHENE SYNTHASE FARNESOL COMPLEX

1DGP の概要

• エントリーDOI: 10.2210/pdb1dgp/pdb
• 関連するPDBエントリー: 1DI1
• 分子名称: ARISTOLOCHENE SYNTHASE, (2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-ol, (2Z,6Z)-3,7,11-trimethyldodeca-2,6,10-trien-1-ol, ... (4 entities in total)
• 機能のキーワード: sesquiterpene cyclase, isoprenoid biosynthesis, lyase
• 由来する生物種: Penicillium roqueforti
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 69883.89

構造登録者

Caruthers, J.M.,Kang, I.,Cane, D.E.,Christianson, D.W. (登録日: 1999-11-24, 公開日: 2001-02-07, 最終更新日: 2024-02-07)

主引用文献

Caruthers, J.M.,Kang, I.,Rynkiewicz, M.J.,Cane, D.E.,Christianson, D.W.
Crystal structure determination of aristolochene synthase from the blue cheese mold, Penicillium roqueforti.
J.Biol.Chem., 275:25533-25539, 2000

PubMed Abstract: The 2.5-A resolution crystal structure of recombinant aristolochene synthase from the blue cheese mold, Penicillium roqueforti, is the first of a fungal terpenoid cyclase. The structure of the enzyme reveals active site features that participate in the cyclization of the universal sesquiterpene cyclase substrate, farnesyl diphosphate, to form the bicyclic hydrocarbon aristolochene. Metal-triggered carbocation formation initiates the cyclization cascade, which proceeds through multiple complex intermediates to yield one exclusive structural and stereochemical isomer of aristolochene. Structural homology of this fungal cyclase with plant and bacterial terpenoid cyclases, despite minimal amino acid sequence identity, suggests divergence from a common, primordial ancestor in the evolution of terpene biosynthesis.

PubMed: 10825154
DOI: 10.1074/jbc.M000433200

実験手法

X-RAY DIFFRACTION (2.8 Å)