1DD7

MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DOMAIN (DELTA 114) (N-[(1,3-BENZODIOXOL-5-YL)METHYL]-1-[2-(1H-IMIDAZOL-1-YL)PYRIMIDIN-4-YL]-4-(METHOXYCARBONYL)-PIPERAZINE-2-ACETAMIDE COMPLEX

Summary for 1DD7

• Entry DOI: 10.2210/pdb1dd7/pdb
• Related: 1NOC 1NOD 1NOS 1NSI 2NOS
• Descriptor: INDUCIBLE NITRIC OXIDE SYNTHASE, SULFITE ION, PROTOPORPHYRIN IX CONTAINING FE, ... (5 entities in total)
• Functional Keywords: nitric oxide, l-arginine monooxygenase, heme, dimerization, inhibitor, nos, oxidoreductase
• Biological source: Mus musculus (house mouse)
• Total number of polymer chains: 1
• Total formula weight: 46446.42

Authors

Adler, M.,Whitlow, M. (deposition date: 1999-11-08, release date: 2000-03-29, Last modification date: 2024-05-22)

Primary citation

McMillan, K.,Adler, M.,Auld, D.S.,Baldwin, J.J.,Blasko, E.,Browne, L.J.,Chelsky, D.,Davey, D.,Dolle, R.E.,Eagen, K.A.,Erickson, S.,Feldman, R.I.,Glaser, C.B.,Mallari, C.,Morrissey, M.M.,Ohlmeyer, M.H.,Pan, G.,Parkinson, J.F.,Phillips, G.B.,Polokoff, M.A.,Sigal, N.H.,Vergona, R.,Whitlow, M.,Young, T.A.,Devlin, J.J.
Allosteric inhibitors of inducible nitric oxide synthase dimerization discovered via combinatorial chemistry.
Proc.Natl.Acad.Sci.USA, 97:1506-1511, 2000

PubMed Abstract: Potent and selective inhibitors of inducible nitric oxide synthase (iNOS) (EC ) were identified in an encoded combinatorial chemical library that blocked human iNOS dimerization, and thereby NO production. In a cell-based iNOS assay (A-172 astrocytoma cells) the inhibitors had low-nanomolar IC(50) values and thus were >1,000-fold more potent than the substrate-based direct iNOS inhibitors 1400W and N-methyl-l-arginine. Biochemical studies confirmed that inhibitors caused accumulation of iNOS monomers in mouse macrophage RAW 264.7 cells. High affinity (K(d) approximately 3 nM) of inhibitors for isolated iNOS monomers was confirmed by using a radioligand binding assay. Inhibitors were >1,000-fold selective for iNOS versus endothelial NOS dimerization in a cell-based assay. The crystal structure of inhibitor bound to the monomeric iNOS oxygenase domain revealed inhibitor-heme coordination and substantial perturbation of the substrate binding site and the dimerization interface, indicating that this small molecule acts by allosterically disrupting protein-protein interactions at the dimer interface. These results provide a mechanism-based approach to highly selective iNOS inhibition. Inhibitors were active in vivo, with ED(50) values of <2 mg/kg in a rat model of endotoxin-induced systemic iNOS induction. Thus, this class of dimerization inhibitors has broad therapeutic potential in iNOS-mediated pathologies.

PubMed: 10677491
DOI: 10.1073/pnas.97.4.1506

Experimental method

X-RAY DIFFRACTION (2.25 Å)