1DCJ

SOLUTION STRUCTURE OF YHHP, A NOVEL ESCHERICHIA COLI PROTEIN IMPLICATED IN THE CELL DIVISION

1DCJ の概要

• エントリーDOI: 10.2210/pdb1dcj/pdb
• 分子名称: YHHP PROTEIN (1 entity in total)
• 機能のキーワード: alpha-beta sandwich, structural genomics, unknown function
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P0A890
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9105.55

構造登録者

Katoh, E.,Hatta, T.,Shindo, H.,Mizuno, T.,Yamazaki, T. (登録日: 1999-11-05, 公開日: 2001-07-18, 最終更新日: 2024-05-22)

主引用文献

Katoh, E.,Hatta, T.,Shindo, H.,Ishii, Y.,Yamada, H.,Mizuno, T.,Yamazaki, T.
High precision NMR structure of YhhP, a novel Escherichia coli protein implicated in cell division.
J.Mol.Biol., 304:219-229, 2000

PubMed Abstract: YhhP, a small protein of 81 amino acid residues encoded by the yhhP gene in the Escherichia coli database, is implicated in cell division although the precise biological function of this protein has not been yet identified. A variety of microorganisms have similar proteins, all of which contain a common CPxP sequence motif in the N-terminal region. We have determined the three-dimensional solution structure of YhhP by NMR spectroscopy in order to obtain insight into its biological function. It folds into a two-layered alpha/beta-sandwich structure with a betaalphabetaalphabetabeta fold, comprising a mixed four-stranded beta-sheet stacked against two alpha-helices, both of which are nearly parallel to the strands of the beta-sheet. The CPxP motif plays a significant structural role in stabilizing the first helix as a part of the new type N-capping box where the Cys-Pro peptide bond adopts a cis configuration. The structure of YhhP displays a striking resemblance to the C-terminal ribosome-binding domain of translation initiation factor IF3 (IF3C). In addition, the surface charge distribution of the RNA-recognition helix of IF3C is nearly the same as that of the corresponding helix of YhhP. These results suggest a structure-based hypothesis in which binding to an RNA target plays an essential role in the function of this ubiquitous protein.

PubMed: 11080457
DOI: 10.1006/jmbi.2000.4170

実験手法

SOLUTION NMR