1DC1

RESTRICTION ENZYME BSOBI/DNA COMPLEX STRUCTURE: ENCIRCLEMENT OF THE DNA AND HISTIDINE-CATALYZED HYDROLYSIS WITHIN A CANONICAL RESTRICTION ENZYME FOLD

1DC1 の概要

• エントリーDOI: 10.2210/pdb1dc1/pdb
• 分子名称: DNA (5'-D(*T*AP*TP*AP*CP*TP*CP*GP*AP*GP*TP*AP*T)-3'), BSOBI RESTRICTION ENDONUCLEASE, 1,4-DIETHYLENE DIOXIDE, ... (4 entities in total)
• 機能のキーワード: protein-dna complex, restriction endonuclease, thermophilic enzyme, degenerate dna recognition, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Geobacillus stearothermophilus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 82297.85

構造登録者

van der Woerd, M.J.,Pelletier, J.J.,Xu, S.-Y.,Friedman, A.M. (登録日: 1999-11-04, 公開日: 2001-02-21, 最終更新日: 2024-02-07)

主引用文献

van der Woerd, M.J.,Pelletier, J.J.,Xu, S.,Friedman, A.M.
Restriction enzyme BsoBI-DNA complex: a tunnel for recognition of degenerate DNA sequences and potential histidine catalysis.
Structure, 9:133-144, 2001

PubMed Abstract: Restriction endonucleases form a diverse family of proteins with substantial variation in sequence, structure, and interaction with recognition site DNA. BsoBI is a thermophilic restriction endonuclease that exhibits both base-specific and degenerate recognition within the sequence CPyCGPuG.

PubMed: 11250198
DOI: 10.1016/S0969-2126(01)00564-0

実験手法

X-RAY DIFFRACTION (1.7 Å)