1DBQ

DNA-BINDING REGULATORY PROTEIN

1DBQ の概要

• エントリーDOI: 10.2210/pdb1dbq/pdb
• 分子名称: PURINE REPRESSOR, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: transcription regulation, dna-binding regulatory protein, purine repressor
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 65053.42

構造登録者

Schumacher, M.A.,Choi, K.Y.,Lu, F.,Zalkin, H.,Brennan, R.G. (登録日: 1996-02-13, 公開日: 1996-12-07, 最終更新日: 2024-02-07)

主引用文献

Schumacher, M.A.,Choi, K.Y.,Lu, F.,Zalkin, H.,Brennan, R.G.
Mechanism of corepressor-mediated specific DNA binding by the purine repressor.
Cell(Cambridge,Mass.), 83:147-155, 1995

PubMed Abstract: The modulation of the affinity of DNA-binding proteins by small molecule effectors for cognate DNA sites is common to both prokaryotes and eukaryotes. However, the mechanisms by which effector binding to one domain affects DNA binding by a distal domain are poorly understood structurally. In initial studies to provide insight into the mechanism of effector-modulated DNA binding of the lactose repressor family, we determined the crystal structure of the purine repressor bound to a corepressor and purF operator. To extend our understanding, we have determined the structure of the corepressor-free corepressor-binding domain of the purine repressor at 2.2 A resolution. In the unliganded state, structural changes in the corepressor-binding pocket cause each subunit to rotate open by as much as 23 degrees, the consequences of which are the disengagement of the minor groove-binding hinge helices and repressor-DNA dissociation.

PubMed: 7553867
DOI: 10.1016/0092-8674(95)90243-0

実験手法

X-RAY DIFFRACTION (2.2 Å)