1DBB

THREE-DIMENSIONAL STRUCTURE OF AN ANTI-STEROID FAB' AND PROGESTERONE-FAB' COMPLEX

Summary for 1DBB

• Entry DOI: 10.2210/pdb1dbb/pdb
• Descriptor: IGG1-KAPPA DB3 FAB (LIGHT CHAIN), IGG1-KAPPA DB3 FAB (HEAVY CHAIN), PROGESTERONE (3 entities in total)
• Functional Keywords: immunoglobulin
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 2
• Total formula weight: 47949.71

Authors

Arevalo, J.H.,Wilson, I.A. (deposition date: 1992-11-11, release date: 1993-10-31, Last modification date: 2024-10-30)

Primary citation

Arevalo, J.H.,Stura, E.A.,Taussig, M.J.,Wilson, I.A.
Three-dimensional structure of an anti-steroid Fab' and progesterone-Fab' complex.
J.Mol.Biol., 231:103-118, 1993

PubMed Abstract: The monoclonal anti-progesterone antibody DB3 binds progesterone with nanomolar affinity (Ka approximately 10(9) M-1), suggesting high specificity. However, DB3 also cross-reacts with similar affinity with a subgroup of structurally distinct, progesterone-like steroids. Crystals of the unliganded Fab' and various steroid-Fab' complexes are isomorphous and belong to the hexagonal space group, P6(4)22, with unit cell dimensions of a = b = 135 A, c = 124 A. Structures of free and progesterone-bound Fab' have been determined by X-ray crystallography at 2.7 A resolution using molecular replacement techniques. Progesterone is bound in a hydrophobic pocket formed mainly by the interaction of three complementarity determining regions L1, H2 and H3. The orientation of the ligand in the binding site was aided by both crystallographic and biochemical analyses of substituted steroids. The indole side-chain of TrpH100 of the DB3 has two different conformations, inter-converting "open" and "closed" forms of the antibody combining site. The TrpH100 indole thus appears to be acting as an antibody-derived surrogate ligand for its own hydrophobic binding pocket. These structures provide the first atomic view of how a steroid interacts with a protein and offer a structural explanation for the restriction of the anti-progesterone response to the VGAM3.8 family of VH genes.

PubMed: 8496956
DOI: 10.1006/jmbi.1993.1260

Experimental method

X-RAY DIFFRACTION (2.7 Å)