1DAB

The Structure of Bordetella Pertussis Virulence Factor P.69 Pertactin

Summary for 1DAB

• Entry DOI: 10.2210/pdb1dab/pdb
• Descriptor: P.69 PERTACTIN (2 entities in total)
• Functional Keywords: pertussis beta helix, cell adhesion
• Biological source: Bordetella pertussis
• Cellular location: Pertactin autotransporter: Periplasm . Outer membrane protein P. Pertactin translocator: Cell outer membrane ; Multi-pass membrane protein : P14283
• Total number of polymer chains: 1
• Total formula weight: 54627.90

Authors

Emsley, P.,Charles, I.G.,Fairweather, N.F.,Isaacs, N.W. (deposition date: 1999-10-31, release date: 1999-12-15, Last modification date: 2024-02-07)

Primary citation

Emsley, P.,Charles, I.G.,Fairweather, N.F.,Isaacs, N.W.
Structure of Bordetella pertussis virulence factor P.69 pertactin.
Nature, 381:90-92, 1996

PubMed Abstract: A new generation of whooping-cough vaccines contain P.69 pertactin, a surface-exposed domain of an outer membrane protein expressed by the virulent bacterium Bordetella pertussis. This protein is a virulence factor that mediates adhesion to target mammalian cells, a reaction that is in part mediated by an RGD sequence. The X-ray crystal structure of P.69 pertactin has been determined to 2.5 A. The protein fold consists of a 16-stranded parallel beta-helix with a V-shaped cross-section, and is the largest beta-helix known to date. Several between-strand weakly conserved amino-acid repeats form internal and external ladders. The structure appears as a helix from which several loops protrude, which contain sequence motifs associated with the biological activity of the protein. One particular (GGXXP)5 sequence is located directly after the RGD motif, and may mediate interaction with epithelial cells. The carboxy-terminal region of P.69 pertactin incorporates a (PQP)5 motif loop containing the major immunoprotective epitope.

PubMed: 8609998
DOI: 10.1038/381090a0

Experimental method

X-RAY DIFFRACTION (2.5 Å)