1D91

G.T BASE PAIRS IN A DNA HELIX. THE CRYSTAL STRUCTURE OF D(G-G-G-G-T-C-C-C)

1D91 の概要

• エントリーDOI: 10.2210/pdb1d91/pdb
• 分子名称: DNA (5'-D(*GP*GP*GP*GP*TP*CP*CP*C)-3') (2 entities in total)
• 機能のキーワード: a-dna, double helix, mismatched, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 4887.21

構造登録者

Kneale, G.,Brown, T.,Kennard, O.,Rabinovich, D. (登録日: 1992-10-17, 公開日: 1993-07-15, 最終更新日: 2024-02-07)

主引用文献

Kneale, G.,Brown, T.,Kennard, O.,Rabinovich, D.
G . T base-pairs in a DNA helix: the crystal structure of d(G-G-G-G-T-C-C-C).
J.Mol.Biol., 186:805-814, 1985

PubMed Abstract: The synthetic deoxyoctanucleotide d(G-G-G-G-T-C-C-C) crystallizes as an A-type DNA double helix containing two adjacent G . T base-pair mismatches. The structure has been refined to an R-factor of 14% at 2.1 A resolution with 104 solvent molecules located. The two G . T mismatches adopt the "wobble" form of base-pairing. The mismatched bases are linked by a network of water molecules interacting with the exposed functional groups in both the major and minor grooves. The presence of two mispaired bases in the octamer has surprisingly little effect on the global structure of the helix or the backbone and glycosidic torsional angles. Base stacking around the mismatch is perturbed, but the central G-T step shows particularly good base overlap, which may contribute to the relatively high stability of this oligomer.

PubMed: 4093986
DOI: 10.1016/0022-2836(85)90398-5

実験手法

X-RAY DIFFRACTION (2.1 Å)