1D8K

SOLUTION STRUCTURE OF THE CENTRAL CORE DOMAIN OF TFIIE BETA

1D8K の概要

• エントリーDOI: 10.2210/pdb1d8k/pdb
• 関連するPDBエントリー: 1D8J
• 分子名称: GENERAL TRANSCRIPTION FACTOR TFIIE-BETA (1 entity in total)
• 機能のキーワード: winged helix-turn-helix, riken structural genomics/proteomics initiative, rsgi, structural genomics, gene regulation
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P29084
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9287.70

構造登録者

Okuda, M.,Watanabe, Y.,Okamura, H.,Hanaoka, F.,Ohkuma, Y.,Nishimura, Y.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 1999-10-25, 公開日: 2000-04-26, 最終更新日: 2024-05-22)

主引用文献

Okuda, M.,Watanabe, Y.,Okamura, H.,Hanaoka, F.,Ohkuma, Y.,Nishimura, Y.
Structure of the central core domain of TFIIEbeta with a novel double-stranded DNA-binding surface.
EMBO J., 19:1346-1356, 2000

PubMed Abstract: Human general transcription factor TFIIE consists of two subunits, TFIIEalpha and TFIIEbeta. Recently, TFIIEbeta has been found to bind to the region where the promoter starts to open to be single-stranded upon transcription initiation by RNA polymerase II. Here, the central core domain of human TFIIEbeta (TFIIEbetac) has been identified by a limited proteolysis. This solution structure has been determined by NMR. It consists of three helices with a beta hairpin at the C-terminus, resembling the winged helix proteins. However, TFIIEbetac shows a novel double-stranded DNA-binding activity where the DNA-binding surface locates on the opposite side to the previously reported winged helix motif by forming a positively charged furrow. A model will be proposed that TFIIE stabilizes the preinitiation complex by binding not only to the general transcription factors together with RNA polymerase II but also to the promoter DNA, where double-stranded DNA starts to open to be single-stranded upon activation of the preinitiation complex.

PubMed: 10716934
DOI: 10.1093/emboj/19.6.1346

実験手法

SOLUTION NMR