1D88

CONFORMATIONAL INFLUENCE OF THE RIBOSE 2'-HYDROXYL GROUP: CRYSTAL STRUCTURES OF DNA-RNA CHIMERIC DUPLEXES

1D88 の概要

• エントリーDOI: 10.2210/pdb1d88/pdb
• 分子名称: DNA/RNA (5'-D(*GP*CP*GP*TP*)-R(*AP*)-D(*TP*AP*CP*GP*C)-3') (2 entities in total)
• 機能のキーワード: a-dna/rna, double helix, dna-rna hybrid complex, dna-rna hybrid
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6122.01

構造登録者

Egli, M.,Usman, N.,Rich, A. (登録日: 1992-08-28, 公開日: 1993-07-15, 最終更新日: 2024-02-07)

主引用文献

Egli, M.,Usman, N.,Rich, A.
Conformational influence of the ribose 2'-hydroxyl group: crystal structures of DNA-RNA chimeric duplexes.
Biochemistry, 32:3221-3237, 1993

PubMed Abstract: We have crystallized three double-helical DNA-RNA chimeric duplexes and determined their structures by X-ray crystallography at resolutions between 2 and 2.25 A. The two self-complementary duplexes [r(G)d(CGTATACGC)]2 and [d(GCGT)r(A)d(TACGC)]2, as well as the Okazaki fragment d(GGGTATACGC).r(GCG)d(TATACCC), were found to adopt A-type conformations. The crystal structures are non-isomorphous, and the crystallographic environments for the three chimeras are different. A number of intramolecular interactions of the ribose 2'-hydroxyl groups contribute to the stabilization of the A-conformation. Hydrogen bonds between 2'-hydroxyls and 5'-oxygens or phosphate oxygens, in addition to the previously observed hydrogen bonds to 1'-oxygens of adjacent riboses and deoxyriboses, are observed in the DNA-RNA chimeric duplexes. The crystalline chimeric duplexes do not show a transition between the DNA A- and B-conformations. CD spectra suggest that the Okazaki fragment assumes an A-conformation in solution as well. In this molecule the three RNA residues may therefore lock the complete decamer in the A-conformation. Crystals of an all-DNA strand with the same sequence as the self-complementary chimeras show a morphology which is different from those of the chimera crystals. Moreover, the oligonucleotide does not match any of the sequence characteristics of DNAs usually adopting the A-conformation in the crystalline state (e.g., octamers with short alternating stretches of purines and pyrimidines). In DNA-RNA chimeric duplexes, it is therefore possible that a single RNA residue can drive the conformational equilibrium toward the A-conformation.

PubMed: 7681688
DOI: 10.1021/bi00064a004

実験手法

X-RAY DIFFRACTION (2 Å)