1D7Q

HUMAN TRANSLATION INITIATION FACTOR EIF1A

1D7Q の概要

• エントリーDOI: 10.2210/pdb1d7q/pdb
• NMR情報: BMRB: 4519
• 分子名称: PROTEIN (N-TERMINAL HISTIDINE TAG), TRANSLATION INITIATION FACTOR 1A (2 entities in total)
• 機能のキーワード: ob-fold, beta-barrel, rna-binding protein, gene regulation
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 18081.17

構造登録者

Battiste, J.L.,Pestova, T.V.,Hellen, C.U.T.,Wagner, G. (登録日: 1999-10-19, 公開日: 2000-03-17, 最終更新日: 2024-05-22)

主引用文献

Battiste, J.L.,Pestova, T.V.,Hellen, C.U.,Wagner, G.
The eIF1A solution structure reveals a large RNA-binding surface important for scanning function.
Mol.Cell, 5:109-119, 2000

PubMed Abstract: The translation initiation factor eIF1A is necessary for directing the 43S preinitiation complex from the 5' end of the mRNA to the initiation codon in a process termed scanning. We have determined the solution structure of human eIF1A, which reveals an oligonucleotide-binding (OB) fold and an additional domain. NMR titration experiments showed that eIF1A binds single-stranded RNA oligonucleotides in a site-specific, but non-sequence-specific manner, hinting at an mRNA interaction rather than specific rRNA or tRNA binding. The RNA binding surface extends over a large area covering the canonical OB fold binding site as well as a groove leading to the second domain. Site-directed mutations at multiple positions along the RNA-binding surface were defective in the ability to properly assemble preinitiation complexes at the AUG codon in vitro.

PubMed: 10678173
DOI: 10.1016/S1097-2765(00)80407-4

実験手法

SOLUTION NMR