1D7O

CRYSTAL STRUCTURE OF BRASSICA NAPUS ENOYL ACYL CARRIER PROTEIN REDUCTASE COMPLEXED WITH NAD AND TRICLOSAN

1D7O の概要

• エントリーDOI: 10.2210/pdb1d7o/pdb
• 関連するPDBエントリー: 1ENO
• 分子名称: ENOYL-[ACYL-CARRIER PROTEIN] REDUCTASE (NADH) PRECURSOR, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, TRICLOSAN, ... (4 entities in total)
• 機能のキーワード: triclosan, enoyl reductase, oxidoreductase
• 由来する生物種: Brassica napus (rape)
• 細胞内の位置: Plastid, chloroplast : P80030
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32347.49

構造登録者

Roujeinikova, A.,Levy, C.,Rowsell, S.,Sedelnikova, S.,Baker, P.J.,Minshull, C.A.,Mistry, A.,Colls, J.G.,Camble, R.,Stuitje, A.R.,Slabas, A.R.,Rafferty, J.B.,Pauptit, R.A.,Viner, R.,Rice, D.W. (登録日: 1999-10-19, 公開日: 1999-11-08, 最終更新日: 2024-02-07)

主引用文献

Roujeinikova, A.,Levy, C.W.,Rowsell, S.,Sedelnikova, S.,Baker, P.J.,Minshull, C.A.,Mistry, A.,Colls, J.G.,Camble, R.,Stuitje, A.R.,Slabas, A.R.,Rafferty, J.B.,Pauptit, R.A.,Viner, R.,Rice, D.W.
Crystallographic analysis of triclosan bound to enoyl reductase.
J.Mol.Biol., 294:527-535, 1999

PubMed Abstract: Molecular genetic studies with strains of Escherichia coli resistant to triclosan, an ingredient of many anti-bacterial household goods, have suggested that this compound works by acting as an inhibitor of enoyl reductase (ENR) and thereby blocking lipid biosynthesis. We present structural analyses correlated with inhibition data, on the complexes of E. coli and Brassica napus ENR with triclosan and NAD(+) which reveal how triclosan acts as a site-directed, picomolar inhibitor of the enzyme by mimicking its natural substrate. Elements of both the protein and the nucleotide cofactor play important roles in triclosan recognition, providing an explanation for the factors controlling its tight binding to the enzyme and for the emergence of triclosan resistance.

PubMed: 10610777
DOI: 10.1006/jmbi.1999.3240

実験手法

X-RAY DIFFRACTION (1.9 Å)