1D7N

SOLUTION STRUCTURE ANALYSIS OF THE MASTOPARAN WITH DETERGENTS

Summary for 1D7N

• Entry DOI: 10.2210/pdb1d7n/pdb
• Descriptor: PROTEIN (WASP VENOM PEPTIDE (MASTOPARAN)) (1 entity in total)
• Functional Keywords: sodium dodecyl sulfate bound conformation, immune system, toxin
• Biological source: Vespula lewisii
• Total number of polymer chains: 1
• Total formula weight: 1480.92

Authors

Hori, Y.,Demura, M.,Iwadate, M.,Niidome, T.,Aoyagi, H.,Asakura, T. (deposition date: 1999-10-19, release date: 2001-06-20, Last modification date: 2024-10-09)

Primary citation

Hori, Y.,Demura, M.,Iwadate, M.,Ulrich, A.S.,Niidome, T.,Aoyagi, H.,Asakura, T.
Interaction of mastoparan with membranes studied by 1H-NMR spectroscopy in detergent micelles and by solid-state 2H-NMR and 15N-NMR spectroscopy in oriented lipid bilayers.
Eur.J.Biochem., 268:302-309, 2001

PubMed Abstract: Several complementary NMR approaches were used to study the interaction of mastoparan, a 14-residue peptide toxin from wasp venom, with lipid membranes. First, the 3D structure of mastoparan was determined using 1H-NMR spectroscopy in perdeuterated (SDS-d25) micelles. NOESY experiments and distance geometry calculations yielded a straight amphiphilic alpha-helix with high-order parameters, and the chemical shifts of the amide protons showed a characteristic periodicity of 3-4 residues. Secondly, solid-state 2H-NMR spectoscopy was used to describe the binding of mastoparan to lipid bilayers, composed of headgroup-deuterated dimyristoylglycerophosphocholine (DMPC-d4) and dimyristoylphosphatidylglycerol (DMPG). By correlating the deuterium quadrupole splittings of the alpha-segments and beta-segments, it was possible to differentiate the electrostatically induced structural response of the choline headgroup from dynamic effects induced by the peptide. A partial phase separation was observed, leading to a DMPG-rich phase and a DMPG-depleted phase, each containing some mastoparan. Finally, the insertion and orientation of a specifically 15N-labeled mastoparan (at position Ala10) in the bilayer environment was investigated by solid-state 15N-NMR spectroscopy, using macroscopically oriented samples. Two distinct orientational states were observed for the mastoparan helix, namely an in-plane and a trans-membrane alignment. The two populations of 90% in-plane and 10% trans-membrane helices are characterized by a mosaic spread of +/- 30 degrees and +/- 10 degrees, respectively. The biological activity of mastoparan is discussed in terms of a pore-forming model, as the peptide is known to be able to induce nonlamellar phases and facilitate a flip-flop between the monolayers.

PubMed: 11168364
DOI: 10.1046/j.1432-1033.2001.01880.x

Experimental method

SOLUTION NMR