1D76

CRYSTAL AND MOLECULAR STRUCTURE OF A DNA FRAGMENT CONTAINING A 2-AMINO ADENINE MODIFICATION: THE RELATIONSHIP BETWEEN CONFORMATION, PACKING, AND HYDRATION IN Z-DNA HEXAMERS

Summary for 1D76

• Entry DOI: 10.2210/pdb1d76/pdb
• Descriptor: DNA (5'-D(*CP*GP*UP*(1AP)P*CP*G)-3') (2 entities in total)
• Functional Keywords: z-dna, double helix, modified, dna
• Total number of polymer chains: 2
• Total formula weight: 3620.41

Authors

Schneider, B.,Ginell, S.L.,Jones, R.,Gaffney, B.,Berman, H.M. (deposition date: 1992-05-19, release date: 1992-09-15, Last modification date: 2024-02-07)

Primary citation

Schneider, B.,Ginell, S.L.,Jones, R.,Gaffney, B.,Berman, H.M.
Crystal and molecular structure of a DNA fragment containing a 2-aminoadenine modification: the relationship between conformation, packing, and hydration in Z-DNA hexamers.
Biochemistry, 31:9622-9628, 1992

PubMed Abstract: The crystal and molecular structure of d(CGUA'CG)2 (where A' is 2-aminoadenine) has been determined and refined to an R factor of 13.8% for data 8.0-1.3 A. The structure is very similar to the original Z-DNA structures with the sequence d(CGCGCG)2 [Gessner, R. V., Frederick, C. A., Quigley, G. J., Rich, A., & Wang, A. H.-J. (1989) J. Biol. Chem. 264, 7921] and shows that the substitution of 2-aminoadenine-uracil base pairs in the two central steps is consistent with Z-DNA formation. In addition, we show how waters mediating intermolecular interactions may help to explain the ZI-ZII conformational pattern found in many Z-DNA structures.

PubMed: 1390739
DOI: 10.1021/bi00155a014

Experimental method

X-RAY DIFFRACTION (1.3 Å)