1D65

MOLECULAR STRUCTURE OF THE B-DNA DODECAMER D(CGCAAATTTGCG)2; AN EXAMINATION OF PROPELLER TWIST AND MINOR-GROOVE WATER STRUCTURE AT 2.2 ANGSTROMS RESOLUTION

1D65 の概要

• エントリーDOI: 10.2210/pdb1d65/pdb
• 分子名称: DNA (5'-D(*CP*GP*CP*AP*AP*AP*TP*TP*TP*GP*CP*G)-3') (2 entities in total)
• 機能のキーワード: b-dna, double helix, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7324.81

構造登録者

Edwards, K.J.,Brown, D.G.,Spink, N.,Neidle, S. (登録日: 1992-03-02, 公開日: 1993-01-15, 最終更新日: 2024-02-07)

主引用文献

Edwards, K.J.,Brown, D.G.,Spink, N.,Skelly, J.V.,Neidle, S.
Molecular structure of the B-DNA dodecamer d(CGCAAATTTGCG)2. An examination of propeller twist and minor-groove water structure at 2.2 A resolution.
J.Mol.Biol., 226:1161-1173, 1992

PubMed Abstract: The crystal structure of the dodecanucleotide duplex d(CGCAAATTTGCG)2 has been solved to 2.2 A resolution and refined to an R-factor of 18.1% with the inclusion of 71 water molecules. The structure shows propeller twists of up to -20 degrees for the A.T base-pairs, although there is probably only one (weak) three-centre hydrogen bond in the six base-pair AT narrow minor-groove region. An extensive ribbon of hydration has been located in this groove that has features distinctive from the classic "spine of hydration". Solvation around phosphate groups is described, with several instances of water molecules bridging between phosphates.

PubMed: 1518049
DOI: 10.1016/0022-2836(92)91059-X

実験手法

X-RAY DIFFRACTION (2.2 Å)