1D5G

SOLUTION STRUCTURE OF THE PDZ2 DOMAIN FROM HUMAN PHOSPHATASE HPTP1E COMPLEXED WITH A PEPTIDE

1D5G の概要

• エントリーDOI: 10.2210/pdb1d5g/pdb
• 関連するPDBエントリー: 3PDZ
• NMR情報: BMRB: 4516
• 分子名称: HUMAN PHOSPHATASE HPTP1E, PEPTIDE FADSEADENEQVSAV (2 entities in total)
• 機能のキーワード: protein-peptide complex, hydrolase
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton (By similarity): Q12923
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 11630.84

構造登録者

Kozlov, G.,Gehring, K.,Ekiel, I. (登録日: 1999-10-07, 公開日: 2002-07-24, 最終更新日: 2024-05-22)

主引用文献

Kozlov, G.,Banville, D.,Gehring, K.,Ekiel, I.
Solution Structure of the PDZ2 Domain from Cytosolic Human Phosphatase hPTP1E Complexed with a Peptide Reveals Contribution of the beta2-beta3 Loop to PDZ Domain-Ligand Interactions
J.Mol.Biol., 320:813-820, 2002

PubMed Abstract: The solution structure of the second PDZ domain from human phosphatase hPTP1E in complex with a C-terminal peptide from the guanine nucleotide exchange factor RA-GEF-2 has been determined using 2D and 3D heteronuclear NMR experiments. Compared to previously solved structures, the hPTP1E complex shows an enlarged interaction surface with the C terminus of the bound peptide. Novel contacts were found between the long structured beta2/beta3 loop of the PDZ domain and the sixth amino acid residue from the C terminus of the peptide. This work underlines the importance of the beta2/beta3 loop for ligand selection by PDZ domains.

PubMed: 12095257
DOI: 10.1016/S0022-2836(02)00544-2

実験手法

SOLUTION NMR