1D24

CRYSTAL AND MOLECULAR STRUCTURE OF A DNA DUPLEX CONTAINING THE CARCINOGENIC LESION O6-METHYLGUANINE

Summary for 1D24

• Entry DOI: 10.2210/pdb1d24/pdb
• Descriptor: DNA (5'-D(*CP*GP*CP*(6OG)P*CP*G)-3') (2 entities in total)
• Functional Keywords: z-dna, double helix, modified, dna
• Total number of polymer chains: 2
• Total formula weight: 3648.46

Authors

Ginell, S.L.,Kuzmich, S.,Jones, R.A.,Berman, H.M. (deposition date: 1990-08-29, release date: 1992-04-07, Last modification date: 2024-02-07)

Primary citation

Ginell, S.L.,Kuzmich, S.,Jones, R.A.,Berman, H.M.
Crystal and molecular structure of a DNA duplex containing the carcinogenic lesion O6-methylguanine.
Biochemistry, 29:10461-10465, 1990

PubMed Abstract: The crystal and molecular structure of the first DNA duplex containing the carcinogenic lesion O6MeG has been determined to a resolution of 1.9 A and refined to an R factor of 19%. (d[CGC-(O6Me)GCG])2 crystallizes in the left-handed Z DNA form and has crystal parameters and conformational features similar to those of the parent sequence [d(CG)3]2. The methyl groups on O6 of G4 and G10 have C5-C6-O6-O6Me torsion angles of 73 degrees and 56 degrees, respectively, and protrude onto the major groove surface. The base-pairing conformation for the methylated G.C base pairs is of the Watson-Crick type as opposed to a wobble-type conformation that had been proposed in a B DNA fragment. As in other Z DNA structures, a spine of hydration is seen in the minor groove.

PubMed: 2271657
DOI: 10.1021/bi00498a005

Experimental method

X-RAY DIFFRACTION (1.9 Å)