1D1R

NMR SOLUTION STRUCTURE OF THE PRODUCT OF THE E. COLI YCIH GENE.

1D1R の概要

• エントリーDOI: 10.2210/pdb1d1r/pdb
• 分子名称: HYPOTHETICAL 11.4 KD PROTEIN YCIH IN PYRF-OSMB INTERGENIC REGION (1 entity in total)
• 機能のキーワード: alpha-beta plait, open-faced beta sandwich, ferredoxin-like fold, structural genomics
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12488.43

構造登録者

Cort, J.R.,Koonin, E.V.,Bash, P.A.,Kennedy, M.A. (登録日: 1999-09-20, 公開日: 1999-10-06, 最終更新日: 2024-05-22)

主引用文献

Cort, J.R.,Koonin, E.V.,Bash, P.A.,Kennedy, M.A.
A phylogenetic approach to target selection for structural genomics: solution structure of YciH.
Nucleic Acids Res., 27:4018-4027, 1999

PubMed Abstract: Structural genomics presents an enormous challenge with up to 100 000 protein targets in the human genome alone. At current rates of structure deter-mination, judicious selection of targets is necessary. Here, a phylogenetic approach to target selection is described which makes use of the National Center for Biotechnology Information database of Clusters of Orthologous Groups (COGS). The strategy is designed so that each new protein structure is likely to provide novel sequence-fold information. To demonstrate this approach, the NMR solution structure of YciH (COG0023), a putative translation initiation factor from Escherichia coli, has been determined and its fold classified. YciH is an ortholog of eIF-1/SUI1, an integral component of the translation initiation complex in eukaryotes. The structure consists of two antiparallel alpha-helices packed against the same side of a five-stranded beta-sheet. The first 31 residues of the 11.5 kDa protein are unstructured in solution. Comparative analysis indicates that the folded portion of YciH resembles a number of structures with the alpha-beta plait topology, though its sequence is not homologous to any of them. Thus, the phylogenetic approach to target selection described here was used successfully to identify a new homologous superfamily within this topology.

PubMed: 10497266
DOI: 10.1093/nar/27.20.4018

実験手法

SOLUTION NMR