1CZD

CRYSTAL STRUCTURE OF THE PROCESSIVITY CLAMP GP45 FROM BACTERIOPHAGE T4

1CZD の概要

• エントリーDOI: 10.2210/pdb1czd/pdb
• 関連するPDBエントリー: 1axc 1plq 1plr 2pol
• 分子名称: DNA POLYMERASE ACCESSORY PROTEIN G45 (1 entity in total)
• 機能のキーワード: bacteriophage t4, processivity clamp, dna replication, ring-shaped protein, gene regulation
• 由来する生物種: Enterobacteria phage T4
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 74643.67

構造登録者

Moarefi, I.,Jeruzalmi, D.,Turner, J.,O'Donnell, M.,Kuriyan, J. (登録日: 1999-09-02, 公開日: 2000-03-03, 最終更新日: 2024-02-07)

主引用文献

Moarefi, I.,Jeruzalmi, D.,Turner, J.,O'Donnell, M.,Kuriyan, J.
Crystal structure of the DNA polymerase processivity factor of T4 bacteriophage.
J.Mol.Biol., 296:1215-1223, 2000

PubMed Abstract: The protein encoded by gene 45 of T4 bacteriophage (gene 45 protein or gp45), is responsible for tethering the catalytic subunit of T4 DNA Polymerase to DNA during high-speed replication. Also referred to as a sliding DNA clamp, gp45 is similar in its function to the processivity factors of bacterial and eukaryotic DNA polymerases, the beta-clamp and PCNA, respectively. Crystallographic analysis has shown that the beta-clamp and PCNA form highly symmetrical ring-shaped structures through which duplex DNA can be threaded. Gp45 shares no sequence similarity with beta-clamp or PCNA, and sequence comparisons have not been able to establish whether it adopts a similar structure. We have determined the crystal structure of gp45 from T4 bacteriophage at 2.4 A resolution, using multiple isomorphous replacement. The protein forms a trimeric ring-shaped assembly with overall dimensions that are similar to those of the bacterial and eukaryotic processivity factors. Each monomer of gp45 contains two domains that are very similar in chain fold to those of beta-clamp and PCNA. Despite an overall negative charge, the inner surface of the ring is in a region of positive electrostatic potential, consistent with a mechanism in which DNA is threaded through the ring.

PubMed: 10698628
DOI: 10.1006/jmbi.1999.3511

実験手法

X-RAY DIFFRACTION (2.45 Å)