1CZ6

SOLUTION STRUCTURE OF ANDROCTONIN

Summary for 1CZ6

• Entry DOI: 10.2210/pdb1cz6/pdb
• Descriptor: PROTEIN (ANDROCTONIN) (1 entity in total)
• Functional Keywords: beta sheet, toxin
• Biological source: Androctonus australis (Sahara scorpion)
• Total number of polymer chains: 1
• Total formula weight: 3088.71

Authors

Mandard, N.,Vovelle, F. (deposition date: 1999-09-01, release date: 2000-01-12, Last modification date: 2024-11-20)

Primary citation

Mandard, N.,Sy, D.,Maufrais, C.,Bonmatin, J.M.,Bulet, P.,Hetru, C.,Vovelle, F.
Androctonin, a novel antimicrobial peptide from scorpion Androctonus australis: solution structure and molecular dynamics simulations in the presence of a lipid monolayer.
J.Biomol.Struct.Dyn., 17:367-380, 1999

PubMed Abstract: Androctonin is a highly cationic antimicrobial peptide from scorpion exhibiting a broad spectrum of activities against bacteria and fungi. It contains 25 amino acids including four cysteine residues forming two disulfide bridges. We report here on the determination of its solution structure by conventional two-dimensional (2D) 1H-NMR spectroscopy and molecular modelling using distance geometry and molecular dynamics methods. The structure of androctonin involves a well-defined highly twisted anti-parallel beta-sheet with strands connected by a more variable positively charged turn. A comparison with the structure of tachyplesin I (horseshoe crab) reveals that the amphiphilic character of the protein surface of this homologous peptide is not observed in androctonin. We have undertaken a 200-ps molecular dynamics simulation study on a system including one androctonin molecule and a monolayer of DMPG (1,2-dimyristoylphosphatidylglycerol) lipids. On the basis of this simulation, the first steps of the membrane permeabilization process are discussed.

PubMed: 10563585

Experimental method

SOLUTION NMR