1CXK

COMPLEX BETWEEN A MALTONONAOSE SUBSTRATE AND BACILLUS CIRCULANS STRAIN 251 CGTASE E257Q/D229N

1CXK の概要

• エントリーDOI: 10.2210/pdb1cxk/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900001 PRD_900010
• 分子名称: PROTEIN (CYCLODEXTRIN-GLYCOSYLTRANSFERASE), alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: glycosyl hydrolase family 13, alpha-amylase family, substrate complex, maltononaose, transferase
• 由来する生物種: Bacillus circulans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 77806.41

構造登録者

Uitdehaag, J.C.M.,Kalk, K.H.,Dijkstra, B.W. (登録日: 1999-02-24, 公開日: 1999-05-03, 最終更新日: 2024-11-13)

主引用文献

Uitdehaag, J.C.,Mosi, R.,Kalk, K.H.,van der Veen, B.A.,Dijkhuizen, L.,Withers, S.G.,Dijkstra, B.W.
X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family.
Nat.Struct.Biol., 6:432-436, 1999

PubMed Abstract: Cyclodextrin glycosyltransferase (CGTase) is an enzyme of the alpha-amylase family, which uses a double displacement mechanism to process alpha-linked glucose polymers. We have determined two X-ray structures of CGTase complexes, one with an intact substrate at 2.1 A resolution, and the other with a covalently bound reaction intermediate at 1.8 A resolution. These structures give evidence for substrate distortion and the covalent character of the intermediate and for the first time show, in atomic detail, how catalysis in the alpha-amylase family proceeds by the concerted action of all active site residues.

PubMed: 10331869
DOI: 10.1038/8235

実験手法

X-RAY DIFFRACTION (2.09 Å)