1CWW

SOLUTION STRUCTURE OF THE CASPASE RECRUITMENT DOMAIN (CARD) FROM APAF-1

1CWW の概要

• エントリーDOI: 10.2210/pdb1cww/pdb
• 分子名称: APOPTOTIC PROTEASE ACTIVATING FACTOR 1 (1 entity in total)
• 機能のキーワード: helical bundle, apoptosis
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: O14727
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11511.16

構造登録者

Day, C.L.,Dupont, C.,Lackmann, M.,Vaux, D.L.,Hinds, M.G. (登録日: 1999-08-26, 公開日: 2000-01-21, 最終更新日: 2024-05-22)

主引用文献

Day, C.L.,Dupont, C.,Lackmann, M.,Vaux, D.L.,Hinds, M.G.
Solution structure and mutagenesis of the caspase recruitment domain (CARD) from Apaf-1.
Cell Death Differ., 6:1125-1132, 1999

PubMed Abstract: Activation of procaspase-9, a key component of the apoptosis mechanism, requires the interaction of its caspase recruitment domain (CARD) with the CARD in the adaptor protein Apaf-1. Using nuclear magnetic resonance spectroscopy and mutagenesis we have determined the structure of the CARD from Apaf-1 and the residues important for binding the CARD in procaspase-9. Apaf-1's CARD contains seven short alpha-helices with the core six helices arranged in an antiparallel manner. Residues in helix 2 have a central role in mediating interaction with procaspase-9 CARD. This interaction surface is distinct from that proposed based on the structure of the CARD from RAIDD, but is coincident with that of the structurally similar FADD death effector domain and the Apaf-1 CARD interface identified by crystallographic studies.

PubMed: 10578182
DOI: 10.1038/sj.cdd.4400584

実験手法

SOLUTION NMR