1CVJ

X-RAY CRYSTAL STRUCTURE OF THE POLY(A)-BINDING PROTEIN IN COMPLEX WITH POLYADENYLATE RNA

1CVJ の概要

• エントリーDOI: 10.2210/pdb1cvj/pdb
• 分子名称: 5'-R(*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3', POLYADENYLATE BINDING PROTEIN 1, ADENOSINE MONOPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: rrm, protein-rna complex, gene regulation-rna complex, gene regulation/rna
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 203097.05

構造登録者

Deo, R.C.,Bonanno, J.B.,Sonenberg, N.,Burley, S.K. (登録日: 1999-08-23, 公開日: 1999-10-04, 最終更新日: 2024-05-29)

主引用文献

Deo, R.C.,Bonanno, J.B.,Sonenberg, N.,Burley, S.K.
Recognition of polyadenylate RNA by the poly(A)-binding protein.
Cell(Cambridge,Mass.), 98:835-845, 1999

PubMed Abstract: The cocrystal structure of human poly(A)-binding protein (PABP) has been determined at 2.6 A resolution. PABP recognizes the 3' mRNA poly(A) tail and plays critical roles in eukaryotic translation initiation and mRNA stabilization/degradation. The minimal PABP used in this study consists of the N-terminal two RRM-type RNA-binding domains connected by a short linker (RRM1/2). These two RRMs form a continuous RNA-binding trough, lined by an antiparallel beta sheet backed by four alpha helices. The polyadenylate RNA adopts an extended conformation running the length of the molecular trough. Adenine recognition is primarily mediated by contacts with conserved residues found in the RNP motifs of the two RRMs. The convex dorsum of RRM1/2 displays a phylogenetically conserved hydrophobic/acidic portion, which may interact with translation initiation factors and regulatory proteins.

PubMed: 10499800
DOI: 10.1016/S0092-8674(00)81517-2

実験手法

X-RAY DIFFRACTION (2.6 Å)