1CUN

CRYSTAL STRUCTURE OF REPEATS 16 AND 17 OF CHICKEN BRAIN ALPHA SPECTRIN

1CUN の概要

• エントリーDOI: 10.2210/pdb1cun/pdb
• 分子名称: PROTEIN (ALPHA SPECTRIN) (2 entities in total)
• 機能のキーワード: two repeats of spectrin, alpha helical linker region, 2 tandem 3-helix coiled- coils, structural protein
• 由来する生物種: Gallus gallus (chicken)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 73150.12

構造登録者

Grum, V.L.,Li, D.,MacDonald, R.I.,Mondragon, A. (登録日: 1999-08-20, 公開日: 1999-10-06, 最終更新日: 2024-02-07)

主引用文献

Grum, V.L.,Li, D.,MacDonald, R.I.,Mondragon, A.
Structures of two repeats of spectrin suggest models of flexibility.
Cell(Cambridge,Mass.), 98:523-535, 1999

PubMed Abstract: Spectrin is a vital component of the cytoskeleton, conferring flexibility on cells and providing a scaffold for a variety of proteins. It is composed of tandem, antiparallel coiled-coil repeats. We report four related crystal structures at 1.45 A, 2.0 A, 3.1 A, and 4.0 A resolution of two connected repeats of chicken brain alpha-spectrin. In all of the structures, the linker region between adjacent units is alpha-helical without breaks, kinks, or obvious boundaries. Two features observed in the structures are (1) conformational rearrangement in one repeat, resulting in movement of the position of a loop, and (2) varying degrees of bending at the linker region. These features form the basis of two different models of flexibility: a conformational rearrangement and a bending model. These models provide novel atomic details of spectrin flexibility.

PubMed: 10481916
DOI: 10.1016/S0092-8674(00)81980-7

実験手法

X-RAY DIFFRACTION (2 Å)