1CTP

STRUCTURE OF THE MAMMALIAN CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE AND AN INHIBITOR PEPTIDE DISPLAYS AN OPEN CONFORMATION

1CTP の概要

• エントリーDOI: 10.2210/pdb1ctp/pdb
• 分子名称: cAMP-DEPENDENT PROTEIN KINASE, cAMP-dependent protein kinase inhibitor, alpha form, MYRISTIC ACID, ... (4 entities in total)
• 機能のキーワード: transferase(phosphotransferase), transferase-transferase inhibitor complex, transferase/transferase inhibitor
• 由来する生物種: Sus scrofa (pig); 詳細
• 細胞内の位置: Cytoplasm (By similarity): P36887
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 43331.00

構造登録者

Karlsson, R.,Zheng, J.,Xuong, N.H.,Taylor, S.S.,Sowadski, J.M. (登録日: 1993-04-08, 公開日: 1994-01-31, 最終更新日: 2024-10-30)

主引用文献

Karlsson, R.,Zheng, J.,Xuong, N.,Taylor, S.S.,Sowadski, J.M.
Structure of the mammalian catalytic subunit of cAMP-dependent protein kinase and an inhibitor peptide displays an open conformation.
Acta Crystallogr.,Sect.D, 49:381-388, 1993

PubMed Abstract: The crystal structure of a binary complex of the porcine heart catalytic (C) subunit of cAMP-dependent protein kinase (space group P4(1)32; a = 171.5 A) complexed with a di-iodinated peptide inhibitor, PKI(5-24), has been solved and refined to 2.9 A resolution with an overall R of 21.1%. The r.m.s. deviations from ideal bond lengths and angles are 0.022 A and 4.3 degrees. A single isotropic B of 17 A(2) was used for all atoms. The structure solution was carried out initially by molecular replacement of electron density followed by refinement against atomic coordinates from orthorhombic crystals of a binary complex of the mouse recombinant enzyme previously described [Knighton, Zheng, Ten Eyck, Ashford, Xuong, Taylor & Sowadski (1991). Science, 253, 407-414]. The most striking difference between the two crystal structures is a large displacement of the small lobe of the enzyme. In the cubic crystal, the beta-sheet of the small lobe is rotated by 15 degrees and translated by 1.9 A with respect to the orthorhombic crystal. Possible explanations for why this binary complex crystallized in an open conformation in contrast to a similar binary complex of the recombinant enzyme are discussed. This study demonstrates that considerable information about parts of a crystal structure can be obtained without a complete crystal structure analysis. Specifically, the six rigid-group parameters of a poly alanine model of the beta-structure were obtained satisfactorily from a crystal structure by refinement of difference Fourier coefficients based on an approximate partial structure model.

PubMed: 15299513
DOI: 10.1107/S0907444993002306

実験手法

X-RAY DIFFRACTION (2.9 Å)