1CSM

THE CRYSTAL STRUCTURE OF ALLOSTERIC CHORISMATE MUTASE AT 2.2 ANGSTROMS RESOLUTION

1CSM の概要

• エントリーDOI: 10.2210/pdb1csm/pdb
• 分子名称: CHORISMATE MUTASE, TRYPTOPHAN (3 entities in total)
• 機能のキーワード: isomerase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60010.90

構造登録者

Xue, Y.,Lipscomb, W.N. (登録日: 1994-08-22, 公開日: 1995-09-15, 最終更新日: 2024-02-07)

主引用文献

Xue, Y.,Lipscomb, W.N.,Graf, R.,Schnappauf, G.,Braus, G.
The crystal structure of allosteric chorismate mutase at 2.2-A resolution.
Proc.Natl.Acad.Sci.USA, 91:10814-10818, 1994

PubMed Abstract: The crystal structure of an allosteric chorismate mutase, the Thr-226-->Ile mutant, from yeast Saccharomyces cerevisiae has been determined to 2.2-A resolution by using the multiple isomorphous replacement method. Solvent-flattening and electron-density modification were applied for phase improvement. The current crystallographic R factor is 0.196. The final model includes 504 of the 512 residues and 97 water molecules. In addition, two tryptophan molecules were identified in the interface between monomers. The overall structure is completely different from the reported structure of chorismate mutase from Bacillus subtilis. This structure showed 71% helices with essentially no beta-sheet structures.

PubMed: 7971967
DOI: 10.1073/pnas.91.23.10814

実験手法

X-RAY DIFFRACTION (2.2 Å)