1CS9

SOLUTION STRUCTURE OF CGGIRGERA IN CONTACT WITH THE MONOCLONAL ANTIBODY MAB 4X11, NMR, 7 STRUCTURES

Summary for 1CS9

• Entry DOI: 10.2210/pdb1cs9/pdb
• Related: 1CT6
• Descriptor: HISTONE H3 PEPTIDE (1 entity in total)
• Functional Keywords: synthetic peptide, tr-noe, antigen-antibody complex, dna binding protein
• Total number of polymer chains: 1
• Total formula weight: 920.05

Authors

Phan Chan Du, A.,Petit, M.C.,Guichard, G.,Briand, J.P.,Muller, S.,Cung, M.T. (deposition date: 1999-08-18, release date: 1999-09-02, Last modification date: 2024-05-22)

Primary citation

Phan-Chan-Du, A.,Petit, M.C.,Guichard, G.,Briand, J.P.,Muller, S.,Cung, M.T.
Structure of antibody-bound peptides and retro-inverso analogues. A transferred nuclear Overhauser effect spectroscopy and molecular dynamics approach.
Biochemistry, 40:5720-5727, 2001

PubMed Abstract: The three-dimensional structures of the two L-peptides, H-CGGIRGERA-OH, called L(A), and H-CGGIRGERG-OH, called L(G), corresponding or close to the IRGERA sequence present in the C-terminal region (residues 130-135) of histone H3, and their retro-inverso analogues HO-mAreGriGGC-NH2, called RI(mA), and HO-mGreGriGGC-NH2, called RI(mG), have been studied by two-dimensional 1H NMR and molecular dynamics calculations in association with a monoclonal antibody generated against L(A). At 25 degrees C, the affinity constants of the monoclonal antibody with respect to RI(mA) and RI(mG) were 75- and 270-fold higher than those measured with the homologous L(A) and L(G) peptides, respectively. Due to the spontaneous epimerization of the mA malonic residue, RI(mA) gave rise to two sets of resonances. With regard to the NH amide region, one set was similar to that for RI(mG) while the second was similar to those for the parent L-peptides L(A) and L(G). The antibody-bound conformations of the two couples of L- and retro-inverso peptides have been analyzed using molecular modeling calculations based on the transferred NOE interproton distances. Folded structures appeared in both cases with a type II' beta-turn in the parent GGIR sequence and a type I' beta-turn in the retro-inverso reGr sequence.

PubMed: 11341837
DOI: 10.1021/bi001151h

Experimental method

SOLUTION NMR