1CRN

WATER STRUCTURE OF A HYDROPHOBIC PROTEIN AT ATOMIC RESOLUTION. PENTAGON RINGS OF WATER MOLECULES IN CRYSTALS OF CRAMBIN

1CRN の概要

• エントリーDOI: 10.2210/pdb1crn/pdb
• 分子名称: CRAMBIN (1 entity in total)
• 機能のキーワード: plant seed protein, plant protein
• 由来する生物種: Crambe hispanica subsp. abyssinica
• 細胞内の位置: Secreted: P01542
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4738.45

構造登録者

Hendrickson, W.A.,Teeter, M.M. (登録日: 1981-04-30, 公開日: 1981-07-28, 最終更新日: 2024-10-30)

主引用文献

Teeter, M.M.
Water structure of a hydrophobic protein at atomic resolution: Pentagon rings of water molecules in crystals of crambin.
Proc.Natl.Acad.Sci.Usa, 81:6014-6018, 1984

PubMed Abstract: The water structure has been analyzed for a model of the protein crambin refined against 0.945-A x-ray diffraction data. Crystals contain 32% solvent by volume, and 77% of the solvent molecules have been located-i.e., 2 ethanol molecules and 64 water molecules with 10-14 alternate positions. Many water oxygen atoms found form chains between polar groups on the surface of the protein. However, a cluster of pentagonal arrays made up of 16 water molecules sits at a hydrophobic, intermolecular cleft and forms a cap around the methyl group of leucine-18. Several waters in the cluster are hydrogen-bonded directly to the protein. Additional closed circular arrays, which include both protein atoms and other water oxygen atoms, form next to the central cluster. This water array stretches in the b lattice direction between groups of three ionic side chains.

PubMed: 16593516
DOI: 10.1073/pnas.81.19.6014

実験手法

X-RAY DIFFRACTION (1.5 Å)