1CR8

LOW DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN COMPLEMENT REPEAT 8

1CR8 の概要

• エントリーDOI: 10.2210/pdb1cr8/pdb
• NMR情報: BMRB: 4475
• 分子名称: PROTEIN (LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN), CALCIUM ION (2 entities in total)
• 機能のキーワード: receptor, ligand binding, calcium binding, ldlr, lrp, lipid binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Low-density lipoprotein receptor-related protein 1 85 kDa subunit: Cell membrane; Single-pass type I membrane protein. Low-density lipoprotein receptor-related protein 1 515 kDa subunit: Cell membrane; Peripheral membrane protein; Extracellular side. Low-density lipoprotein receptor-related protein 1 intracellular domain: Cytoplasm: Q07954
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4696.21

構造登録者

Huang, W.,Dolmer, K.,Gettins, P.G.W. (登録日: 1998-12-14, 公開日: 1998-12-23, 最終更新日: 2024-10-30)

主引用文献

Huang, W.,Dolmer, K.,Gettins, P.G.
NMR solution structure of complement-like repeat CR8 from the low density lipoprotein receptor-related protein.
J.Biol.Chem., 274:14130-14136, 1999

PubMed Abstract: The low density lipoprotein receptor-related protein is a member of the low density lipoprotein receptor family and contains clusters of cysteine-rich complement-like repeats of about 42 residues that are present in all members of this family of receptors. These clusters are thought to be the principal binding sites for protein ligands. We have expressed one complement-like repeat, CR8, from the cluster in lipoprotein receptor-related protein that binds certain proteinase inhibitor-proteinase complexes and used three-dimensional NMR on the 13C/15N-labeled protein to determine the structure in solution of the calcium-bound form. The structure is very similar in overall fold to repeat 5 from the low density lipoprotein receptor (LB5), with backbone root mean square deviation of 1.5 A. The calcium-binding site also appears to be homologous, with four carboxyl and two backbone carbonyl ligands. However, differences in primary structure are such that equivalent surfaces that might represent the binding interfaces are very different from one another, indicating that different domains will have very different ligand specificities.

PubMed: 10318830
DOI: 10.1074/jbc.274.20.14130

実験手法

SOLUTION NMR