1CQ9

PEANUT LECTIN-TRICLINIC FORM

1CQ9 の概要

• エントリーDOI: 10.2210/pdb1cq9/pdb
• 関連するPDBエントリー: 2PEL
• 分子名称: PROTEIN (PEANUT LECTIN), CALCIUM ION, MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: lectin, legume lectin, open quaternary structure, triclinic form, acidic ph
• 由来する生物種: Arachis hypogaea (peanut)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 101215.88

構造登録者

Ravishankar, R.,Suguna, K.,Surolia, A.,Vijayan, M. (登録日: 1999-08-06, 公開日: 2002-05-01, 最終更新日: 2023-08-09)

主引用文献

Ravishankar, R.,Thomas, C.J.,Suguna, K.,Surolia, A.,Vijayan, M.
Crystal structures of the peanut lectin-lactose complex at acidic pH: retention of unusual quaternary structure, empty and carbohydrate bound combining sites, molecular mimicry and crystal packing directed by interactions at the combining site.
Proteins, 43:260-270, 2001

PubMed Abstract: The crystal structures of a monoclinic and a triclinic form of the peanut lectin-lactose complex, grown at pH 4.6, have been determined. They contain two and one crystallographically independent tetramers, respectively. The unusual "open" quaternary structure of the lectin, observed in the orthorhombic complex grown in neutral pH, is retained at the acidic pH. The sugar molecule is bound to three of the eight subunits in the monoclinic crystals, whereas the combining sites in four are empty. The lectin-sugar interactions are almost the same at neutral and acidic pH. A comparison of the sugar-bound and free subunits indicates that the geometry of the combining site is relatively unaffected by ligand binding. The combining site of the eighth subunit in the monoclinic crystals is bound to a peptide stretch in a loop from a neighboring molecule. The same interaction exists in two subunits of the triclinic crystals, whereas density corresponding to sugar exists in the combining sites of the other two subunits. Solution studies show that oligopeptides with sequences corresponding to that in the loop bind to the lectin at acidic pH, but only with reduced affinity at neutral pH. The reverse is the case with the binding of lactose to the lectin. A comparison of the neutral and acidic pH crystal structures indicates that the molecular packing in the latter is directed to a substantial extent by the increased affinity of the peptide loop to the combining site at acidic pH.

PubMed: 11288176
DOI: 10.1002/prot.1037

実験手法

X-RAY DIFFRACTION (3.5 Å)