1CNU

PHOSPHORYLATED ACTOPHORIN FROM ACANTAMOEBA POLYPHAGA

1CNU の概要

• エントリーDOI: 10.2210/pdb1cnu/pdb
• 分子名称: ACTOPHORIN (2 entities in total)
• 機能のキーワード: actin-binding protein, adf, cofilin, contractile
• 由来する生物種: Acanthamoeba polyphaga
• 細胞内の位置: Cytoplasm: P37167
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15524.37

構造登録者

Blanchoin, L.,Robinson, R.C.,Choe, S.,Pollard, T.D. (登録日: 1999-05-24, 公開日: 1999-06-01, 最終更新日: 2024-11-06)

主引用文献

Blanchoin, L.,Pollard, T.D.
Interaction of actin monomers with Acanthamoeba actophorin (ADF/cofilin) and profilin.
J.Biol.Chem., 273:25106-25111, 1998

PubMed Abstract: Acanthamoeba actophorin is a member of ADF/cofilin family that binds both actin monomers and filaments. We used fluorescence anisotropy to study the interaction of actin monomers with recombinant actophorin labeled with rhodamine on a cysteine substituted for Serine-88. Labeled actophorin retains its affinity for actin and ability to reduce the low shear viscosity of actin filaments. At physiological ionic strength, actophorin binds Mg-ADP-actin monomers (Kd = 0.1 microM) 40 times stronger than Mg-ATP-actin monomers. When bound to actin monomers, actophorin has no effect on elongation at either end of actin filaments by Mg-ATP-actin and slightly increases the rate of elongation at both ends by Mg-ADP-actin. Thus actophorin does not sequester actin monomers. Sedimentation equilibrium ultracentrifugation shows that actophorin and profilin compete for binding actin monomers. Actophorin and profilin have opposite effects on the rate of exchange of nucleotide bound to actin monomers. Despite the high affinity of actophorin for ADP-actin, physiological concentrations of profilin overcome the inhibition of ADP exchange by actophorin. Profilin rapidly recycles ADP-actin back to the profilin-ATP-actin pool ready for elongation of actin filaments.

PubMed: 9737968
DOI: 10.1074/jbc.273.39.25106

実験手法

X-RAY DIFFRACTION (2.25 Å)