1CN0

CRYSTAL STRUCTURE OF D(ACCCT)

1CN0 の概要

• エントリーDOI: 10.2210/pdb1cn0/pdb
• 分子名称: DNA (5'-D(*AP*CP*CP*CP*T)-3') (2 entities in total)
• 機能のキーワード: telomere, i-motif, dna
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 5759.95

構造登録者

Weil, J.,Min, T.,Cheng, Y.,Wang, S.,Sutherland, C.,Sinha, N.,Kang, C. (登録日: 1999-05-24, 公開日: 2000-05-24, 最終更新日: 2023-08-02)

主引用文献

Weil, J.,Min, T.,Yang, C.,Wang, S.,Sutherland, C.,Sinha, N.,Kang, C.
Stabilization of the i-motif by intramolecular adenine-adenine-thymine base triple in the structure of d(ACCCT).
Acta Crystallogr.,Sect.D, 55:422-429, 1999

PubMed Abstract: The crystal structure of d(ACCCT), solved by molecular replacement, shows a four-stranded i-motif conformation, where two parallel duplexes intercalate with one another in opposite orientations. Each duplex is stabilized by hemi-protonated C-C+ base pairing between parallel strands, and a string of water molecules bridge the cytosine N4 atoms to phosphate O atoms. This structure of d(ACCCT) shows examples of reversed Hoogsteen and Watson-Crick base pairing in both intermolecular and intramolecular manners to stabilize the tetraplex. Noticeably, the four-stranded complex is further stabilized at one end by a three-base hydrogen-bonding network, in which two adenines and a thymine form four hydrogen bonds via a reverse Hoogsteen and an asymmetric adenine-adenine base pairing. The structure of d(ACCCT) shows a similar local structure to that found in the d(TAA) part of the crystal structure of d(TAACCC) and provides further structural evidence that these base arrangements are essential for stabilizing these novel DNA super-secondary structures.

PubMed: 10089350
DOI: 10.1107/S0907444998012529

実験手法

X-RAY DIFFRACTION (2.2 Å)