1CKO

STRUCTURE OF MRNA CAPPING ENZYME IN COMPLEX WITH THE CAP ANALOG GPPPG

1CKO の概要

• エントリーDOI: 10.2210/pdb1cko/pdb
• 分子名称: MRNA CAPPING ENZYME, ZINC ION, DIGUANOSINE-5'-TRIPHOSPHATE (3 entities in total)
• 機能のキーワード: mrna, capping enzyme, nucleotidyltransferase
• 由来する生物種: Paramecium bursaria Chlorella virus 1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38738.81

構造登録者

Hakansson, K.,Wigley, D.B. (登録日: 1997-09-06, 公開日: 1998-01-28, 最終更新日: 2024-05-22)

主引用文献

Hakansson, K.,Wigley, D.B.
Structure of a complex between a cap analogue and mRNA guanylyl transferase demonstrates the structural chemistry of RNA capping.
Proc.Natl.Acad.Sci.USA, 95:1505-1510, 1998

PubMed Abstract: Paramecium bursaria Chlorella virus PBCV-1 mRNA guanylyl transferase (capping enzyme) has been complexed with an mRNA cap analogue G[5']ppp[5']G and crystallized. The crystals belong to space group C2221 with unit cell dimensions a = 78.4 A, b = 164.1 A, c = 103.3 A, and diffraction data to 3.1 A has been collected by using synchrotron radiation. The structure has been solved by molecular replacement by using each of the two domains in the previously determined structure of the enzyme in complex with GTP. The conformation is open with respect to the active site cleft, and all contacts between enzyme and ligand are mediated by domain 1. One of the guanine bases is bound in the same pocket that is utilized by GTP. The conformation of the ligand positions the beta phosphate and the active site lysine on opposite sides of the alpha phosphate. This geometry is optimal for nucleophilic substitution reactions and has previously been found for GTP in the closed conformational form of the capping enzyme, where the lysine can be guanylylated upon treatment with excess manganese(II) ions. The remainder of the cap analogue runs along the conserved active site Lys82 Thr83 Asp84 Gly85 Ile86 Arg87 motif, and the second guanine, corresponding to the 5' RNA base, is stacked against the hydrophobic Ile86. The ligand displays approximate 2-fold symmetry with intramolecular hydrogen bonding between the 2' and 3' hydroxyls of the two ribose rings.

PubMed: 9465045
DOI: 10.1073/pnas.95.4.1505

実験手法

X-RAY DIFFRACTION (3.1 Å)