1CJ5

BOVINE BETA-LACTOGLOBULIN A

1CJ5 の概要

• エントリーDOI: 10.2210/pdb1cj5/pdb
• 分子名称: BETA-LACTOGLOBULIN A (1 entity in total)
• 機能のキーワード: beta-lactoglobulin a, dynamics, transport protein
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18395.20

構造登録者

Kuwata, K.,Hoshino, M.,Forge, V.,Era, S.,Batt, C.A.,Goto, Y. (登録日: 1999-04-22, 公開日: 2000-04-26, 最終更新日: 2023-12-27)

主引用文献

Kuwata, K.,Hoshino, M.,Forge, V.,Era, S.,Batt, C.A.,Goto, Y.
Solution structure and dynamics of bovine beta-lactoglobulin A.
Protein Sci., 8:2541-2545, 1999

PubMed Abstract: Using heteronuclear NMR spectroscopy, we studied the solution structure and dynamics of bovine beta-lactoglobulin A at pH 2.0 and 45 degrees C, where the protein exists as a monomeric native state. The monomeric NMR structure, comprising an eight-stranded continuous antiparallel beta-barrel and one major alpha-helix, is similar to the X-ray dimeric structure obtained at pH 6.2, including betaI-strand that forms the dimer interface and loop EF that serves as a lid of the interior hydrophobic hole. [1H]-15N NOE revealed that betaF, betaG, and betaH strands buried under the major alpha-helix are rigid on a pico- to nanosecond time scale and also emphasized rapid fluctuations of loops and the N- and C-terminal regions.

PubMed: 10595563

実験手法

SOLUTION NMR