1CGT

STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE REFINED AT 2.0 ANGSTROMS RESOLUTION

Summary for 1CGT

• Entry DOI: 10.2210/pdb1cgt/pdb
• Descriptor: CYCLODEXTRIN GLYCOSYL-TRANSFERASE, CALCIUM ION (3 entities in total)
• Functional Keywords: glycosyltransferase
• Biological source: Bacillus circulans
• Cellular location: Secreted (By similarity): P30920
• Total number of polymer chains: 1
• Total formula weight: 74645.20

Authors

Klein, C.,Schulz, G.E. (deposition date: 1992-06-10, release date: 1994-01-31, Last modification date: 2024-11-06)

Primary citation

Klein, C.,Schulz, G.E.
Structure of cyclodextrin glycosyltransferase refined at 2.0 A resolution.
J.Mol.Biol., 217:737-750, 1991

PubMed Abstract: The previously reported structural model of cyclodextrin glycosyltransferase (EC 2.4.1.19) from Bacillus circulans has been improved. For this purpose the known sequence was built into an electron density map established by multiple isomorphous replacement and subsequent solvent-flattening at 2.5 A resolution. The resulting model was refined at 2.0 A resolution using a simulated annealing refinement method. Based on 70,171 independent reflections in the range 7.0 to 2.0 A resolution, a final R-factor of 17.6% was obtained with a model obeying standard geometry within 0.013 A in bond lengths and 2.7 degrees in bond angles. The final model consists of all 684 amino acid residues, two calcium ions and 588 solvent molecules.

PubMed: 1826034
DOI: 10.1016/0022-2836(91)90530-J

Experimental method

X-RAY DIFFRACTION (2 Å)