1CGP

CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

Summary for 1CGP

• Entry DOI: 10.2210/pdb1cgp/pdb
• Descriptor: DNA (5'-D(*GP*CP*GP*AP*AP*AP*AP*GP*TP*GP*TP*GP*AP*CP*AP*TP*AP*T)-3'), DNA (5'-D(*GP*TP*CP*AP*CP*AP*CP*TP*TP*TP*TP*CP*G)-3'), PROTEIN (CATABOLITE GENE ACTIVATOR PROTEIN (CAP)), ... (4 entities in total)
• Functional Keywords: protein-dna complex, double helix, transcription-dna complex, transcription/dna
• Biological source: Escherichia coli; More
• Total number of polymer chains: 6
• Total formula weight: 65796.28

Authors

Schultz, S.C.,Shields, G.C.,Steitz, T.A. (deposition date: 1991-08-12, release date: 1994-01-31, Last modification date: 2024-02-07)

Primary citation

Schultz, S.C.,Shields, G.C.,Steitz, T.A.
Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees.
Science, 253:1001-1007, 1991

PubMed Abstract: The 3 angstrom resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with a 30-base pair DNA sequence shows that the DNA is bent by 90 degrees. This bend results almost entirely from two 40 degrees kinks that occur between TG/CA base pairs at positions 5 and 6 on each side of the dyad axis of the complex. DNA sequence discrimination by CAP derives both from sequence-dependent distortion of the DNA helix and from direct hydrogen-bonding interactions between three protein side chains and the exposed edges of three base pairs in the major groove of the DNA. The structure of this transcription factor--DNA complex provides insights into possible mechanisms of transcription activation.

PubMed: 1653449

Experimental method

X-RAY DIFFRACTION (3 Å)