1CGP

CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

1CGP の概要

• エントリーDOI: 10.2210/pdb1cgp/pdb
• 分子名称: DNA (5'-D(*GP*CP*GP*AP*AP*AP*AP*GP*TP*GP*TP*GP*AP*CP*AP*TP*AP*T)-3'), DNA (5'-D(*GP*TP*CP*AP*CP*AP*CP*TP*TP*TP*TP*CP*G)-3'), PROTEIN (CATABOLITE GENE ACTIVATOR PROTEIN (CAP)), ... (4 entities in total)
• 機能のキーワード: protein-dna complex, double helix, transcription-dna complex, transcription/dna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 65796.28

構造登録者

Schultz, S.C.,Shields, G.C.,Steitz, T.A. (登録日: 1991-08-12, 公開日: 1994-01-31, 最終更新日: 2024-02-07)

主引用文献

Schultz, S.C.,Shields, G.C.,Steitz, T.A.
Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees.
Science, 253:1001-1007, 1991

PubMed Abstract: The 3 angstrom resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with a 30-base pair DNA sequence shows that the DNA is bent by 90 degrees. This bend results almost entirely from two 40 degrees kinks that occur between TG/CA base pairs at positions 5 and 6 on each side of the dyad axis of the complex. DNA sequence discrimination by CAP derives both from sequence-dependent distortion of the DNA helix and from direct hydrogen-bonding interactions between three protein side chains and the exposed edges of three base pairs in the major groove of the DNA. The structure of this transcription factor--DNA complex provides insights into possible mechanisms of transcription activation.

PubMed: 1653449

実験手法

X-RAY DIFFRACTION (3 Å)