1CFR

CRYSTAL STRUCTURE OF CITROBACTER FREUNDII RESTRICTION ENDONUCLEASE CFR10I AT 2.15 ANGSTROMS RESOLUTION.

Summary for 1CFR

Entry DOI	10.2210/pdb1cfr/pdb
Descriptor	RESTRICTION ENDONUCLEASE (2 entities in total)
Functional Keywords	restriction endonuclease, restriction enzyme
Biological source	Citrobacter freundii
Total number of polymer chains	1
Total formula weight	32051.39
Authors	Bozic, D.,Grazulis, S.,Siksnys, V.,Huber, R. (deposition date: 1996-02-02, release date: 1997-01-27, Last modification date: 2024-02-07)
Primary citation	Bozic, D.,Grazulis, S.,Siksnys, V.,Huber, R. Crystal structure of Citrobacter freundii restriction endonuclease Cfr10I at 2.15 A resolution. J.Mol.Biol., 255:176-186, 1996 Cited by PubMed Abstract: The X-ray crystal structure of Citrobacter freundii restriction endonuclease Cfr10I has been determined at a resolution of 2.15 A by multiple isomorphous replacement methods and refined to an R-factor of 19.64%. The structure of Cfr10I represents the first structure of a restriction endonuclease recognizing a degenerated nucleotide sequence. Structural comparison of Cfr10I with previously solved structures of other restriction enzymes suggests that recognition of specific sequence occurs through contacts in the major and the minor grooves of DNA. The arrangement of the putative active site residues shows some striking differences from previously described restriction endonucleases and supports a two-metal-ion mechanism of catalysis. PubMed: 8568865 DOI: 10.1006/jmbi.1996.0015 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.15 Å)

Structure validation