1CFR

CRYSTAL STRUCTURE OF CITROBACTER FREUNDII RESTRICTION ENDONUCLEASE CFR10I AT 2.15 ANGSTROMS RESOLUTION.

1CFR の概要

• エントリーDOI: 10.2210/pdb1cfr/pdb
• 分子名称: RESTRICTION ENDONUCLEASE (2 entities in total)
• 機能のキーワード: restriction endonuclease, restriction enzyme
• 由来する生物種: Citrobacter freundii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32051.39

構造登録者

Bozic, D.,Grazulis, S.,Siksnys, V.,Huber, R. (登録日: 1996-02-02, 公開日: 1997-01-27, 最終更新日: 2024-02-07)

主引用文献

Bozic, D.,Grazulis, S.,Siksnys, V.,Huber, R.
Crystal structure of Citrobacter freundii restriction endonuclease Cfr10I at 2.15 A resolution.
J.Mol.Biol., 255:176-186, 1996

PubMed Abstract: The X-ray crystal structure of Citrobacter freundii restriction endonuclease Cfr10I has been determined at a resolution of 2.15 A by multiple isomorphous replacement methods and refined to an R-factor of 19.64%. The structure of Cfr10I represents the first structure of a restriction endonuclease recognizing a degenerated nucleotide sequence. Structural comparison of Cfr10I with previously solved structures of other restriction enzymes suggests that recognition of specific sequence occurs through contacts in the major and the minor grooves of DNA. The arrangement of the putative active site residues shows some striking differences from previously described restriction endonucleases and supports a two-metal-ion mechanism of catalysis.

PubMed: 8568865
DOI: 10.1006/jmbi.1996.0015

実験手法

X-RAY DIFFRACTION (2.15 Å)