1CFC

CALCIUM-FREE CALMODULIN

Summary for 1CFC

• Entry DOI: 10.2210/pdb1cfc/pdb
• Descriptor: CALMODULIN (1 entity in total)
• Functional Keywords: calcium-binding protein
• Biological source: Xenopus laevis (African clawed frog)
• Total number of polymer chains: 1
• Total formula weight: 16721.35

Authors

Kuboniwa, H.,Tjandra, N.,Grzesiek, S.,Ren, H.,Klee, C.B.,Bax, A. (deposition date: 1995-08-02, release date: 1995-12-07, Last modification date: 2024-05-22)

Primary citation

Kuboniwa, H.,Tjandra, N.,Grzesiek, S.,Ren, H.,Klee, C.B.,Bax, A.
Solution structure of calcium-free calmodulin.
Nat.Struct.Biol., 2:768-776, 1995

PubMed Abstract: The three-dimensional structure of calmodulin in the absence of Ca2+ has been determined by three- and four-dimensional heteronuclear NMR experiments, including ROE, isotope-filtering combined with reverse labelling, and measurement of more than 700 three-bond J-couplings. In analogy with the Ca(2+)-ligated state of this protein, it consists of two small globular domains separated by a flexible linker, with no stable, direct contacts between the two domains. In the absence of Ca2+, the four helices in each of the two globular domains form a highly twisted bundle, capped by a short anti-parallel beta-sheet. This arrangement is qualitatively similar to that observed in the crystal structure of the Ca(2+)-free N-terminal domain of troponin C.

PubMed: 7552748
DOI: 10.1038/nsb0995-768

Experimental method

SOLUTION NMR