1CF5

BETA-MOMORCHARIN STRUCTURE AT 2.55 A

1CF5 の概要

• エントリーDOI: 10.2210/pdb1cf5/pdb
• 分子名称: PROTEIN (BETA-MOMORCHARIN), beta-D-xylopyranose-(1-2)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-3)]2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: ribosome-inactivating protein, rna n-glycosidase activity, glycoprotein
• 由来する生物種: Momordica charantia (balsam pear)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58299.67

構造登録者

Yuan, Y.-R.,He, Y.-N.,Xiong, J.-P.,Xia, Z.-X. (登録日: 1999-03-24, 公開日: 1999-06-07, 最終更新日: 2024-11-20)

主引用文献

Yuan, Y.R.,He, Y.N.,Xiong, J.P.,Xia, Z.X.
Three-dimensional structure of beta-momorcharin at 2.55 A resolution.
Acta Crystallogr.,Sect.D, 55:1144-1151, 1999

PubMed Abstract: Beta-Momorcharin (Mr approximately 29 kDa) is a single-chained ribosome-inactivating protein (RIP) with a branched hexasaccharide bound to Asn51. The crystal structure of beta-momorcharin has been determined using the molecular-replacement method and refined to 2. 55 A resolution. The final structural model gave an R factor of 17. 2% and root-mean-square deviations of 0.016 A and 1.76 degrees from ideal bond lengths and bond angles, respectively. beta-Momorcharin contains nine alpha-helices, two 310 helices and three beta-sheets, and its overall structure is similar to those of other single-chained RIPs. Residues Tyr70, Tyr109, Glu158 and Arg161 are expected to define the active site of beta-momorcharin as an rRNA N-glycosidase. The oligosaccharide is linked to the protein through an N-glycosidic bond, beta-GlcNAc-(1-N)-Asn51, and stretches from the surface of the N-terminal domain far from the active site, which suggests that it should not play a role in enzymatic function. The oligosaccharide of each beta-momorcharin molecule interacts with the protein through hydrogen bonds, although in the crystals most of these are intermolecular interactions with the protein atoms in an adjacent unit cell. This is the first example of an RIP structure which provides information about the three-dimensional structure and binding site of the oligosaccharide in the active chains of RIPs.

PubMed: 10329776
DOI: 10.1107/S0907444999003297

実験手法

X-RAY DIFFRACTION (2.55 Å)