1CDP

RESTRAINED LEAST SQUARES REFINEMENT OF NATIVE (CALCIUM) AND CADMIUM-SUBSTITUTED CARP PARVALBUMIN USING X-RAY CRYSTALLOGRAPHIC DATA AT 1.6-ANGSTROMS RESOLUTION

1CDP の概要

• エントリーDOI: 10.2210/pdb1cdp/pdb
• 分子名称: CADMIUM-SUBSTITUTED CALCIUM-BINDING PARVALBUMIN B, CADMIUM ION (3 entities in total)
• 機能のキーワード: calcium binding
• 由来する生物種: Cyprinus carpio (common carp)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11701.65

構造登録者

Swain, A.L.,Kretsinger, R.H.,Amma, E.L. (登録日: 1990-01-24, 公開日: 1990-10-15, 最終更新日: 2024-10-23)

主引用文献

Swain, A.L.,Kretsinger, R.H.,Amma, E.L.
Restrained least squares refinement of native (calcium) and cadmium-substituted carp parvalbumin using X-ray crystallographic data at 1.6-A resolution.
J.Biol.Chem., 264:16620-16628, 1989

PubMed Abstract: Carp parvalbumin coordinates calcium through one carbonyl oxygen atom and the oxygen-containing side chains of 5 amino acid residues, or 4 residues and a water molecule, in a helix-loop-helix structural motif. Other calcium-binding proteins, including calmodulin and troponin C, also possess this unique calcium-binding design, which is designated EF-hand or calmodulin fold. Parvalbumin has two such sites, labeled CD and EF. Each of the calcium-binding sites of refined structures of proteins belonging to this group has a 7-oxygen coordination sphere except those of the structure of parvalbumin as it was reported in 1975. This structure had been refined at 1.9 A using difference Fourier techniques on film data. The CD site appeared to be 6-coordinate and the EF site 8-coordinate. Results of NMR experiments using 113Cd-substituted parvalbumin, however, indicate that the sites are similar to one another with coordination number greater than 6. To resolve the inconsistency between crystallographic and NMR results, 1.6 A area detector data was collected for native and cadmium-substituted parvalbumin; the structures have been refined to R factors of 18.7% and 16.4%, respectively, with acceptable geometry and low errors in atomic coordinates. Differences between the parvalbumin structure described in 1975 and the present structure are addressed, including the discovery of 7-coordination for both the CD and EF sites.

PubMed: 2777802

実験手法

X-RAY DIFFRACTION (1.6 Å)