1C9A

SOLUTION STRUCTURE OF NEUROMEDIN B

1C9A の概要

• エントリーDOI: 10.2210/pdb1c9a/pdb
• 分子名称: NEUROMEDIN B (1 entity in total)
• 機能のキーワード: neuromedin b, hormone-growth factor complex, hormone/growth factor
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1132.30

構造登録者

Lee, S.,Kim, Y. (登録日: 1999-08-01, 公開日: 1999-11-11, 最終更新日: 2024-10-30)

主引用文献

Lee, S.,Kim, Y.
Solution structure of neuromedin B by (1)H nuclear magnetic resonance spectroscopy.
FEBS Lett., 460:263-269, 1999

PubMed Abstract: The solution structure of neuromedin B (NMB) was investigated using two-dimensional nuclear magnetic resonance (NMR) spectroscopy in membrane-mimicking environments. NMB adopts a relaxed helical conformation from Trp(4) to Met(10) in 50% aqueous 2,2, 2-trifluoroethanol (TFE) solution and in 150 mM SDS micelles. Sidechain atoms of the three residues, Trp(4), His(8) and Phe(9) orient toward the same direction and these residues might play a key role on interacting with hydrophobic acyl chains of the phospholipids in the membrane. NOESY experiments performed on NMB in non-deuterated SDS micelle show that aromatic ring protons of Trp(4) and Phe(9) residues are in close contact with methylene protons of SDS micelles. In addition, proton longitudinal relaxation data proved that the interactions between NMB with SDS micelle are characterized as extrinsic interaction. Trp(4) and Phe(9) seem to be important in interaction with receptor and this agrees with the previous studies of structure-activity relationship (Howell, D.C. et al. (1996) Int. J. Pept. Protein Res. 48, 522-531). These conformational features might be helpful in understanding the molecular mechanism of the function of NMB and developing the efficient drugs.

PubMed: 10544247
DOI: 10.1016/S0014-5793(99)01346-0

実験手法

SOLUTION NMR