1C8O

2.9 A STRUCTURE OF CLEAVED VIRAL SERPIN CRMA

1C8O の概要

• エントリーDOI: 10.2210/pdb1c8o/pdb
• 分子名称: ICE INHIBITOR (3 entities in total)
• 機能のキーワード: serpin fold, viral protein
• 由来する生物種: Cowpox virus; 詳細
• 細胞内の位置: Host cytoplasm (Potential): P07385 P07385
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37983.54

構造登録者

Simonovic, M.,Gettins, P.G.W.,Volz, K. (登録日: 2000-06-01, 公開日: 2000-09-06, 最終更新日: 2023-12-27)

主引用文献

Simonovic, M.,Gettins, P.G.W.,Volz, K.
Crystal structure of viral serpin crmA provides insights into its mechanism of cysteine proteinase inhibition.
Protein Sci., 9:1423-1427, 2000

PubMed Abstract: CrmA is an unusual viral serpin that inhibits both cysteine and serine proteinases involved in the regulation of host inflammatory and apoptosis processes. It differs from other members of the serpin superfamily by having a reactive center loop that is one residue shorter, and by its apparent inability to form SDS-stable covalent complexes with cysteine proteinases. To obtain insight into the inhibitory mechanism of crmA, we determined the crystal structure of reactive center loop-cleaved crmA to 2.9 A resolution. The structure, which is the first of a viral serpin, suggests that crmA can inhibit cysteine proteinases by a mechanism analogous to that used by other serpins against serine proteinases. However, one striking difference from other serpins, which may be significant for in vivo function, is an additional highly charged antiparallel strand for b sheet A, whose sequence and length are unique to crmA.

PubMed: 10975564

実験手法

X-RAY DIFFRACTION (2.9 Å)