1C5I
HYDROGEN BONDING AND CATALYSIS: AN UNEXPECTED EXPLANATION FOR HOW A SINGLE AMINO ACID SUBSTITUTION CAN CHANGE THE PH OPTIMUM OF A GLYCOSIDASE
Summary for 1C5I
Entry DOI | 10.2210/pdb1c5i/pdb |
Descriptor | ENDO-1,4-BETA-XYLANASE, beta-D-xylopyranose-(1-4)-1,5-anhydro-2-deoxy-2-fluoro-D-xylitol (3 entities in total) |
Functional Keywords | glycosidase, ph-dependent enzyme mechanism, general acid/ base catalysis, isotope shift, short hydrogen bonds, xylan, hydrolase |
Biological source | Bacillus circulans |
Total number of polymer chains | 1 |
Total formula weight | 20678.23 |
Authors | Joshi, M.D.,Sidhu, G.,Pot, I.,Brayer, G.D.,Withers, S.G.,Mcintosh, L.P. (deposition date: 1999-11-24, release date: 2000-05-12, Last modification date: 2023-12-27) |
Primary citation | Joshi, M.D.,Sidhu, G.,Pot, I.,Brayer, G.D.,Withers, S.G.,McIntosh, L.P. Hydrogen bonding and catalysis: a novel explanation for how a single amino acid substitution can change the pH optimum of a glycosidase. J.Mol.Biol., 299:255-279, 2000 Cited by PubMed: 10860737DOI: 10.1006/jmbi.2000.3722 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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