1C5I

HYDROGEN BONDING AND CATALYSIS: AN UNEXPECTED EXPLANATION FOR HOW A SINGLE AMINO ACID SUBSTITUTION CAN CHANGE THE PH OPTIMUM OF A GLYCOSIDASE

1C5I の概要

• エントリーDOI: 10.2210/pdb1c5i/pdb
• 分子名称: ENDO-1,4-BETA-XYLANASE, beta-D-xylopyranose-(1-4)-1,5-anhydro-2-deoxy-2-fluoro-D-xylitol (3 entities in total)
• 機能のキーワード: glycosidase, ph-dependent enzyme mechanism, general acid/ base catalysis, isotope shift, short hydrogen bonds, xylan, hydrolase
• 由来する生物種: Bacillus circulans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20678.23

構造登録者

Joshi, M.D.,Sidhu, G.,Pot, I.,Brayer, G.D.,Withers, S.G.,Mcintosh, L.P. (登録日: 1999-11-24, 公開日: 2000-05-12, 最終更新日: 2023-12-27)

• 主引用文献: Joshi, M.D.,Sidhu, G.,Pot, I.,Brayer, G.D.,Withers, S.G.,McIntosh, L.P.; Hydrogen bonding and catalysis: a novel explanation for how a single amino acid substitution can change the pH optimum of a glycosidase.; J.Mol.Biol., 299:255-279, 2000; PubMed: 10860737; DOI: 10.1006/jmbi.2000.3722

実験手法

X-RAY DIFFRACTION (1.8 Å)